| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Sinorhizobium medicae | TCGCCACAGTTCTGACAGC | ACGTGGCGAGGTTCTTGT | 60.30 | 59.18 | 237 | 117 |
100.00%
|
100.00%
|
| Primer 2 | Sinorhizobium medicae | AAGCCTTCGCCAAGTTGC | AGCAATGTCGGCAGCAGT | 58.96 | 59.97 | 230 | 112 |
100.00%
|
100.00%
|
| Primer 3 | Sinorhizobium medicae | GAAGCCTTCGCCAAGTTGC | AGCAATGTCGGCAGCAGT | 60.08 | 59.97 | 231 | 112 |
100.00%
|
100.00%
|
| Primer 4 | Sinorhizobium medicae | TTCTGCTGGCTGTGGATGG | TTCGCGTCGCTTTTGCTG | 60.00 | 59.75 | 231 | 108 |
100.00%
|
100.00%
|
| Primer 5 | Sinorhizobium medicae | TTTCGTCTCCTCGCTGCTG | AGCCCACAAGAAGCAGCA | 60.08 | 59.48 | 251 | 107 |
100.00%
|
100.00%
|
| Primer 6 | Sinorhizobium medicae | ACACGCTTCGGCTCGAAA | ATGTCGTGCCCATCTGCA | 59.97 | 59.33 | 286 | 106 |
100.00%
|
100.00%
|
| Primer 7 | Sinorhizobium medicae | GCCACAGTTCTGACAGCCA | ACGTGGCGAGGTTCTTGT | 60.23 | 59.18 | 235 | 104 |
100.00%
|
100.00%
|
| Primer 8 | Sinorhizobium medicae | TGGTTGCGCTTCTCGGAT | AGCATCTGCATCAGCGCA | 59.34 | 60.12 | 185 | 100 |
100.00%
|
100.00%
|
| Primer 9 | Sinorhizobium medicae | TGAGCATCGTCGCCAACA | AGTGGGTGAGCGTTTCGAT | 59.66 | 59.33 | 201 | 99 |
100.00%
|
100.00%
|
| Primer 10 | Sinorhizobium medicae | ACACGCTTCGGCTCGAAA | ATAGCCGTTCATCGCCTGG | 59.97 | 59.93 | 183 | 99 |
100.00%
|
100.00%
|